Wheat Fields Case Study

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Assignment: Trouble In the Wheat Fields Case Study
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Assignment: Trouble In the Wheat
Fields Case Study
Mike Brown was a wheat farmer with a reputation for having the best crops in the
region. For decades, he sold his harvest for the highest price to the major
companies in the industry. In recent years, Mike noticed that the neighbouring
farm, operated by John Wilson, was producing bigger wheat plants.
John, who recently took over his father’s farm, was a recent graduate of a
university program in molecular biology. Mike often wondered why a scientist
would be interested in farming. Mike had learned all he knew about farming from
his father and grandfather; he did not understand how some young man who was
interested in test tubes and Bunsen burners could possibly be better at growing
wheat. Mike was determined that next year his crop would be best.
The following harvest, Mike and John both produced tall golden wheat plants that
were almost identical. John, who was using a special transgenic seed, was
concerned that Mike had stolen his seeds. Early that spring, someone broke into
John’s barn and stole a bag of his seed. Mike vehemently denied the theft, but
John was extremely suspicious.
John, wanting to find out if his neighbour was growing his transgenic wheat
plants, hired your laboratory to investigate the incident. John has provided you
with one of his transgenic seeds, one of the non-transgenic seeds and a seed
from Mike’s harvest this year.
Instructions
The table below contains short DNA sequences (36 nucleotides) obtained from
the wheat seed John took from Mike’s farm. Complete Parts I and II described in
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08/05/2022, 00:53
Assignment: Trouble In the Wheat Fields Case Study
the accordion panels. Upload your answers to the assignment questions to the
appropriate Dropbox.
Allele
(Copy)
DNA Sequence
Copy 1
Strand
1: ATGGTCGGCCATAGAGTGGGCCAGTACTTGAGGTAT
Strand
2: TACCAGCCGGTATCTCACCCGGTCATGAACTCCATA
Copy 2
Strand
1: ATGGTCGGCCATAGAGTGGGTCAGTACTTGAGGTAT
Strand
2: TACCAGCCGGTATCTCACCCAGTCATGAACTCCATA
Part I
Cut both copies of the DNA sequence with the restriction endonuclease
Hemophilius aegypticus (HaeIII).
The restriction enzyme HaeIII recognizes the following four nucleotide
sequence:
GG | CC
CC | GG
HaeIII cuts the DNA strand between the guanine (G) and the cytosine (C).
1. Look at each of the two copies of the DNA sequence and identify how many
times you find the restriction site (GGCC). Record the number of HaeIII sites
in the table provided.
2. Once you have identified the number of restriction sites present in each
sequence, determine the sizes of the DNA fragments you get when you cut
the DNA with HaeIII. Add the fragments sizes to your table.
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Assignment: Trouble In the Wheat Fields Case Study
DNA Sequence
Number of
Restriction
DNA
Frag
Sizes
Sites
nucle
Allele Copy 1
Strand 1:
ATGGTCGGCCATAGAGTGGGCCAGTACTTGAGGTAT
Strand 2:
TACCAGCCGGTATCTCACCCGGTCATGAACTCCATA
Allele Copy 2
Strand 1:
ATGGTCGGCCATAGAGTGGGTCAGTACTTGAGGTAT
Strand 2:
TACCAGCCGGTATCTCACCCAGTCATGAACTCCATA
Part II
Questions
Answer the questions below.
1. What conclusions can you draw from the RFLP analysis of the seed taken
from Mike’s harvest tell you?
2. Do the results of the test prove that Mike stole the seed from John?
3. Explain how electrophoresis can separate DNA fragments based on size.
4. In an RFLP analysis, would using multiple restriction enzymes be beneficial to
determining the genetic identity of an individual?
5. Research and discuss how one of the following is an example of
polymorphism in humans:
a. Blood types
b. Male and female sex types
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Assignment: Trouble In the Wheat Fields Case Study
Assessment Details
Your submission should include the following:

Your answers to the questions written in your own words

All in-text citations and a reference list according to the APA style guide for
sources used in your submission
Upload your complete answers to the Assignment: Trouble in the Wheat Field
Case Study Dropbox.
Submit this assignment to the dropbox. This assignment will be evaluated for a grade that will
contribute to your overall final grade in this course.
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