Waldorf University Enterobacter Aerogenes Microbiology Lab Report

microbe Enterobacter Aerogenes. I performed multiple biochemical test to come to the conclusion of my specific microbe. Please see below for my microbe results and attached rubric for paper.

Paper Criteria (please see attached for more details)

Must use paragraph format (write as narrative)

MLA bibliography format

Length of paper 4-5 pages (less than three, not including chart, will not be accepted)

1 ½ line spacing, 11 pt type font, proper grammar, punctuation, spelling, number pages

Refrain from the use of personal pronouns and be specific (minimize fluff)

Names of all genus (first word in microbe name) is capitalized and species (second name) is lower case (e.g., S. aureus)

Names of all taxa must be printed in italics.The first use of the name should be written out with subsequent uses abbreviating the first name (e.g., Staphylococcus aureus andS. aureus)

Chart size should not exceed 1/2 page

Identify/discuss a minimum of 5 experiments that were most helpful in the ID of your microbe

Explain/Interpret what the results mean or indicate

Discuss any variables or reasons why your results may have been contrary to book results

Must reference all quoted materials used

Cannot use class handout materials or Wikipedia as a cited reference

Results!!

-Sugar Fermentation (Lactose, Dextrose, and Sucrose)- positive for both acid and gas production.(Hence “AG” via chart attached)

-Triple suagr iron (TSI)- positive for acid production

-Methyl Red Voges Proskauer (MVRP) Test- MR Reaction was (-) no glucose breakdown /no color change, VP Reaction was (+) glucose breakdown to acetylmethylcarbinol – red ring around top of broth

-Citrate Utilization Test- (+) citrate utilization giving alkaline PH- royal blue media

-Sulfide, Indole, & Motility (SIM Test)- Sulfide- (-) no H2S to FeS/ no color change,Indole (+) indole production cherry red at top of agar, Motility was (+) due to cloudiness throughout agar

-Gelatin (Protein) Hydrolysis- (-) gelatinase was produced medium remains liquid after refrigeration.

-Starch Hydrolysis – (-) no starch hydrolysis agar is blue/blackincluding around bacterial growth

-Casein (Milk) Hydrolysis- (+) casein hydrolyzed clear zone around bacterial growth.

-Lipid Hydrolysis- (-) no lipid , opaque agar

-Urea Hydrolysis- (-) no urea/color change

-Nitrate Reduction- (+) reduced nitrates to nitric oxide, nitrous oxide or nitrogen gas, no color change

-Oxidase Test- (-) no cytochrome oxidase activity/no color change

-Catalase Test- (+) catalase production, bubbles appear

Grading Rubric for Unknown Experiment
I
Structure/Appearance (15pts)
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II
Cover Page (Include Title, Name, Date, Professor, Class)
Scientific format (Introduction, Methods, Results, Discussion/Conclusion, References)
Tables, figures, and photos formatted correctly
Paper neat, tidy, and of publishable quality
Free of spelling, punctuation, grammar, and other errors
Are microorganisms in correct format
Font rules followed, minimum required pages, proper references for quoted materials, etc.
Introduction (15pts)
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III
Paragraph format
General description of the objective for your work, why it is important
Provide brief overview of the investigative work
Brief introduction of organism with background information
Methods (20pts)
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IV
Listing of methods, growth conditions, and equipment
Paraphrasing of method procedures (no cutting and pasting)
Results (20pts)
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V
Using tables, figures, photos, etc.
Clearly describe results of each experiment conducted and interpret their meaning
Identifying those experiments that were most instrumental in the identification of the unknown
Construct chart identifying results in a neat manner comparing columns and organisms
Explain results in paragraph form, organized by test grouping of those that were most in the
identification of the microbe
Discussion/Conclusion (20pts)
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VI
Clearly explain how results were used to identify the microbe
Relate results to physiological characteristics of the microbe
Explanation of experimental errors
Discuss any alternative possible identification of species
Concluding paragraph identifying the microbe
References (10pts)
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List all publications or documentation used and/or referred to in the paper
Minimum of three references
Grading Rubric for Unknown Experiment
Notes:
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Must use paragraph format (write as narrative)
MLA bibliography format
Length of paper 4-5 pages (less than three, not including chart, will not be accepted)
1 ½ line spacing, 11 pt type font, proper grammar, punctuation, spelling, number pages
Refrain from the use of personal pronouns and be specific (minimize fluff)
Names of all genus (first word in microbe name) is capitalized and species (second name) is
lower case (e.g., S. aureus)
Names of all taxa must be printed in italics. The first use of the name s/b written out with
subsequent uses abbreviating the first name (e.g., Staphylococcus aureus and S. aureus)
Chart size should not exceed 1/2 page
Identify/discuss a minimum of 5 experiments that were most helpful in the ID of your microbe
Explain/Interpret what the results mean or indicate
Discuss any variables or reasons why your results may have been contrary to book results
Must reference all quoted materials used
Cannot use class handout materials or Wikipedia as a cited reference