Please help me find the unknown species for my Online microbiology lab class I have uploded pictures and a report.
Here are the instructions my number is 83,
Unknown Lab Home work
You will be completing the Unknown Lab. All students must report Phenol red glucose and phenol red lactose results. When writing results for phenol red, you must write it using the proper way. For instance, you cannot write “yellow with a bubble”. Instead, you would write A/G or A/+. For the interpretations, you will write what does it mean in terms of the biochemical tests. For example, a phenol red glucose broth that is A/G or A/+, mean that the organism ferments glucose with the production of CO2.
\These two tests are mandatory for everyone. After this test, each student will choose which test to run next. You are to write down the rationale for that. Therefore, many tables on that pdf file will be unfilled. If you fill out every table of the document, you will lose points. You are not allowed to report results of every single one listed in the file. For each test that you report, you must use the proper way to record the results, the interpretations for the test, and what’s next including the possible organisms that you may have and why you are running the next test.
After you have identified your unknown organism, you will type the name of the bacterium on top of page 7 of lab report. Then, you will do some research using reliable sources. You will then write about your findings in an format (page 8 of lab report file). All instructions for this part are in page 7 of lab report file. Do not forget to include your citations in the last page of the lab report.
Microbiology Lab
Identification of Unknown Bacteria
Student Name:_____________________
Bacteria ID #: ______________________
Tests:
Record the results of the tests you used to identify your unknown organism. You CANNOT list a
result for every single test, only those which were used to identify your unknown organism.
Skip the sections of the tests that were not necessary to run on your unknown. When recording
results, all results must be recorded properly and in the same manner as shown in lab. Any test
that has more than one result associated with it must have all possible results recorded.
Gram Stain Result: _______________________
Morphology Result: ______________________
Phenol Red Sugar Broth Tests:
*This test is mandatory for everyone and it is the first test that you must run.
Phenol Red Glucose Broth Phenol Red Lactose Broth
Results: Results:
Interpretation of Results: Interpretation of Results:
Based on my Phenol Red Glucose and Lactose results,
the possible organisms are:
The next test that I will run will be:
The reason of what I will run that test is because:
Download file first and save on your computer. Then, fill out the form and save.
TSIA Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Oxidase Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Catalase Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
SIM Test:
*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you
fill this table and it is not necessary. You must record result and interpretation for S, I, and M.
Results for each test in SIM:
Interpretations for each test in SIM:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
MR-VP Test:
*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you
fill this table and it is not necessary. You must record result and interpretation for MR and VP.
Results for each test in MR-VP:
Interpretation for each test in MR-VP:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Citrate Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Gelatinase Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Starch Hydrolysis (Amylase) Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Casease Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
Urease Test:
*Complete this table ONLY if you need it to identify your unknown.
Points will be taken off if you fill this table and it is not necessary.
Results:
Interpretation of Results:
Did you identify your organism?
If you still haven’t identified your organism, list the possible
organisms that you may have based on the results on this test.
Which test will you run next and why?
My unknown organism was identified as: __________________________
If the organism is pathogenic, describe one disease or condition, human or veterinary, that the
identified organism is a pathogen for.
If the organism is non-pathogenic, describe one use of it as tool in biotechnology.
The research for this section should be complete and detailed in essay format and not bullet
points. All sources must be cited using MLA format.
You will type your information on the next page.
Guidelines are as follows:
Disease or condition (IF YOUR UNKNOWN IS PATHOGENIC): Full name of disease, mechanism
of the disease (what does this organism do that causes a disease state in the host), toxins or cell
damage description. Treatment of the disease both supportive and pharmaceutical. Include the
prevalence of the disease in the United States and any programs or research that may be
ongoing for the disease.
Biotechnology Use (IF YOUR UNKNOWN IS NON-PATHOGENIC): What field of biotechnology is
the organism being used in. List the use, what it is being used to produce and what the purpose
and function of that biotech product is. Describe if it is used for industrial or pharmaceutical
purposes. List the company currently using the organism and of the product is already on the
market or still in research.
Essay
Citations in MLA Format