Enzyme Principles

In a hypothetical biochemical laboratory experiment, you are tasked with investigating the kinetics of a catalyzed reaction involving the conversion of substrate S to product P. The reaction is catalyzed by an enzyme, E, and is influenced by various factors. Design and describe a comprehensive experimental protocol to systematically analyze the impact of substrate concentration, enzyme concentration, and temperature on the reaction rate. Your protocol should encompass a range of concentrations and temperatures to provide a thorough understanding of the underlying mechanisms.Part A: Enzyme Concentration Dependencea. Outline a set of experiments to investigate the influence of enzyme concentration on the reaction rate. Provide the rationale behind your chosen concentrations and explain how the experimental design allows for a comprehensive analysis of enzyme kinetics.Part B: Temperature Dependencea. Formulate a plan for examining the impact of temperature on the reaction rate. Specify the temperature range to be investigated and elucidate the potential effects on both enzyme and substrate molecules.Part C: Integration and Analysisa. Synthesize the findings from the experiments on substrate concentration, enzyme concentration, and temperature dependence. Discuss any observed trends or anomalies and relate these observations to the underlying biochemical principles.b. Formulate a comprehensive conclusion that summarizes the key insights gained from the entire experimental series. Address the potential practical implications of the results and propose future research directions based on your findings.