Biology 101 lab report

There is a bio lab write up that needs to be done today.  Please just read the lab write up requirements and look at the picture attached.  Then write up the lab, it is a introduction to biology lab so it is not advanced, just keep it simple college work.  It needs to be one at 5pm today at the latest but I will give a bonus to anyone who can have it done faster. 

1- label all lanes, label marker sizes, and indicate which three lanes, containing at least one BSA sample and one E. coli sample, you are writing about.

2- lanes 2, 5, 6, 9, and 11 are BSA, lanes 14 and 15 are empty, and lanes 3, 4, 7, 8, 10, 12, and 13 are E. coli.

Biology 105 Laboratory Fall 2013
Instructor: Ayça Akal-Strader

Guidelines for Lab Report

Lab 1: Introduction to Biomolecules – A Look at Proteins Using SDS-PAGE

Your report for Lab 1: A Look at Proteins Using SDS-PAGE is due the week of September 30 & October 1/2/3. Please include the following information in your report:

Introduction:

• Background/theory of SDS-PAGE

• Purpose of the experiment

• Expected results

Results:

Note: In addition to the specific data discussed below, your Results section should always include one or more paragraphs of text that provide:

• A brief description of the procedure

• Explanations of any charts, graphs, figures, or calculations that are included

• Statements about the most interesting/noteworthy data

Gel Picture:

• Include your gel picture with enough white space around it to label cleanly. Affix it to a larger sheet of blank paper, if necessary.

• Number the lanes

• Give the picture a title and a caption. The caption should briefly define what is in each lane.

• Using the key provided, label your molecular weight standards.

• In one of your BSA lanes, circle the BSA band and label it with its approximate size. This information should also be included in the written part of your Results section.

• In one of your E. coli lanes, circle the largest and smallest bands you can see clearly, and label them with their approximate sizes. Choose another band you can see clearly, circle it and label it with its approximate size. This information should also be included in the written part of your Results section.

• Estimate the number of bands you see in one of your E. coli lanes. This information does not need to be written on your gel picture; you should include it in the written part of your Results section.

**Note: If you cannot clearly see your own results due to technical problems with the gel, you may analyze another group’s results that are shown on the same gel. If you do this, say so!

Discussion:

• Did your results match your expectations? If not, why not?

• How many different proteins do you think are present in an E. coli? Did you see that many protein bands in your E. coli lane? If not, why not?

• Why are some of your E. coli bands thicker/more intense than others?

Lab Report Rewrites

You may rewrite TWO of your first FIVE lab reports in an effort to improve your grade.

You do not need to rewrite the entire report; just fix the problems that caused you to lose points the first time around.

You MUST hand in the original version of your report along with your corrected version. If you do not have the original attached, we will not accept your rewrite.

Your final grade on the rewritten report will be the average of your original grade and the new grade. (This can only help you. If you somehow manage to get a lower grade on the rewrite, we will keep your original grade as is.)

Rewrites are due the week of November 18/19/20/21, which is also the week of the Wrap Up and Review & the Lab Exam.

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