bio lab report

Okay, these are the details for the second lab report. 
The “guidelines” tells you how to write and what questions should you answe in the paper.
The “lab 2″paper is a background about what is the experiment about,
The “procedure”  is how we did the experiment step by step and the data that i took.
The ” Graphing page” is where you have to graph as what the instructor mentions on the guide line. you can use Excel foe graphing or any other thing but you have to add the graph.
. Add the graph !
And ask me if there is anything you need.

Lamia/Final data ( results) x

1- label all lanes, label marker sizes, and indicate which three lanes, containing at least one BSA sample and one
E. coli sample, you are writing about.

2- lanes 2, 5, 6, 9, and 11 are BSA, lanes 14 and 15 are empty, and lanes 3, 4, 7, 8, 10, 12, and 13 are
E. coli.

image1

Lamia/Graphing page

Lamia/Guidelines

Biology 105 Laboratory Fall 2013
Instructor: Ayça Akal-Strader

Guidelines for Lab Report

Lab 2: Quantification of Protein (Bradford Assay)

Your report for Lab 2: Quantification of Protein (Bradford Assay) is due the week of October 7/8/9/10. Please include the following information in your report:

Hypothesis: as usual

Introduction:

• Background/theory of Bradford Assay

• Purpose of the experiment

Results:

In addition to the specific data discussed below, your Results section should always include one or more paragraphs of text that provide:

• A brief description of the procedure

• Explanations of any charts, graphs, figures, or calculations that are included

• Statements about the most interesting/noteworthy data

Data:

1. Table of measured absorbances (like Table 2 on p. 31).

2. Table showing protein concentrations of unknowns (like Table 3 on p. 31). Say which unknowns—1, 2, or both—you used.

**Please re-make the tables for your report. DO NOT simply tear out p. 31 from your lab manual and staple it to your report.

3. Standard Curve:

• Label with title and caption

• Label axes: x-axis = Concentration (μg/ml); y-axis = Absorbance at 595 nm. Be sure to include units on Concentration. Remember that absorbance (optical density; OD) has no units.

• Plot points, leaving room to plug in your unknown absorbances to find their concentrations

• Connect the dots

(Note: Do NOT draw a straight line—unless your data really looks like a straight line. The samples we measured did not fall into the “linear range” of the spectrophotometer, and everyone’s data that I saw flattened out a lot at the high concentration end of the range. Connect your data points with a curve.)

• Indicate by drawing horizontal and vertical lines how you found the concentration of your unknowns.

Discussion:

• Did your results match your expectations? If not, why not?

• Did you have any difficulty finding the concentration of any of your unknowns?

• Do you think your measurement of protein concentration was accurate? Did your duplicates agree well? For your standards, did your absorbances increase as your protein concentrations increased?

Conclusion: as usual

Lab Report Rewrites

You may rewrite TWO of your first FIVE lab reports in an effort to improve your grade.

You do not need to rewrite the entire report; just fix the problems that caused you to lose points the first time around.

You MUST hand in the original version of your report along with your corrected version. If you do not have the original attached, we will not accept your rewrite.

Your final grade on the rewritten report will be the average of your original grade and the new grade. (This can only help you. If you somehow manage to get a lower grade on the rewrite, we will keep your original grade as is.)

Rewrites are due the week of November 18/19/20/21, which is also the week of the Wrap Up and Review & the Lab Exam.

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