Fillable pdf

Read “Who Kidnapped Fluffy?!!” – the document contains information about the scenario and the chemical principles used in the virtual lab. 

Download/print “Who Kidnapped Fluffy?!! – Data Collection and Analysis.” 

Watch the video below, pausing as needed, and fill in the data table in the lab document, and then write an analysis of the data by answering the questions after the data table.

4

Who Kidnapped Fluffy?!! Virtual Lab

DATA COLLECTION:

Watch the video linked in the Falcon Online Content to fill out this data table. List the
colors and color patterns observed

IN DETAIL

(for example, what color is on top and how do the colors progress down the chromatogram, do they fade, do they get darker, etc. )for each pen (the four reference pens & unknown) from each solvent system as indicated in the table below.

											Colors Observed in Developed Chromatograms
Pen Number:	Acetone-Based Solvent System	Isopropyl Alcohol-Based Solvent System
Unknown
Ref. pen # 1
Ref. pen # 2
Ref. pen # 3
Ref. pen # 4

4
Who Kidnapped Fluffy?!! Virtual Lab

Name

DATA ANALYSIS: (each response for 1 – 4 should be ~2-3 sentences)

For questions 1 – 4,
don’t draw any conclusions, just describe what you saw in the Data Collection! You will explain your reasoning and draw conclusions in #6.

1. Reference Pen #1:

a. Describe the similarities between Reference pen #1 and the Unknown pen in the acetone solvent system.

b. Describe the differences between Reference pen #1 and the Unknown pen in the acetone solvent system.

c. Describe the similarities between Reference pen #1 and the Unknown pen in the alcohol solvent system.

d. Describe the differences between Reference pen #1 and the Unknown pen in the alcohol solvent system.

2. Reference pen #2:

a. Describe the similarities between Reference pen #2 and the Unknown pen in the acetone solvent system.

b. Describe the differences between Reference pen #2 and the Unknown pen in the acetone solvent system.

c. Describe the similarities between Reference pen #2 and the Unknown pen in the alcohol solvent system.

d. Describe the differences between Reference pen #2 and the Unknown pen in the alcohol solvent system.

3. Reference pen #3:

a. Describe the similarities between Reference pen #3 and the Unknown pen in the acetone solvent system.

b. Describe the differences between Reference pen #3 and the Unknown pen in the acetone solvent system.

c. Describe the similarities between Reference pen #3 and the Unknown pen in the alcohol solvent system.

d. Describe the differences between Reference pen #3 and the Unknown pen in the alcohol solvent system.

4. Reference pen #4:

a. Describe the similarities between Reference pen #4 and the Unknown pen in the acetone solvent system.

b. Describe the differences between Reference pen #4 and the Unknown pen in the acetone solvent system.

c. Describe the similarities between Reference pen #4 and the Unknown pen in the alcohol solvent system.

d. Describe the differences between Reference pen #4 and the Unknown pen in the alcohol solvent system.

5. The pen that best matches the Unknown pen is Ref. pen # ________________________. (no extra sentences, just identify the pen)

6. Explain your answer to #5: how did you eliminate three of the four Reference pens as your Unknown pen? How did you decide which pen matched? Explain why each pen is or is not the Unknown pen.
This is where you collect all your data and summarize your conclusion. This answer should be
at least 8 sentences (2 for each pen).

Who Kidnapped Fluffy?!! Virtual Lab

INTRODUCTION AND THEORY:

Physical Separations & Chromatography

Mixtures are defined as materials which contain at least two pure substances, which can be separated by physical means. Pure substances, elements and compounds, CANNOT be separated using physical means (i.e., using a method which does not break any chemical bonds). In this virtual lab, you will use a physical separation method (paper chromatography) to compare different kinds of known inks (which are mixtures) with an ink sample from an unknown source. Through the comparison of your data, you should be able to identify the source of your unknown ink.

Ink is typically a mixture of some sort of
solvent and one or more
colorant materials. The solvent is commonly either water or some sort of oil (e.g., soybean oil), and is used to dissolve the other ink components in the ink mixture. The colorant is the substance used to give color to the ink and can be either a
dye (small molecules which can be dissolved) or a
pigment (much larger molecules which cannot be dissolved). Finally, many inks contain special
additives to improve the properties of the ink (e.g., fungicides, biocides, resins for resilience, buffers to control the pH, and humectants to reduce evaporation of the solvent). When you write with an ink pen, the ink’s solvent evaporates and leaves behind the non-volatile residue. (Source: www.misterinkjet.com)

In order to analyze a mixture such as ink, we need to employ a means of physical separation. One method of physical separation which is ideally suited for analyzing ink is called
paper chromatography. A small amount of the mixture of interest is placed as a dot at the bottom of a strip of special “chromatography” paper. Some type of solvent (which may be a either a single pure substance or a mixture) is then allowed to wick up through the paper and carry along the components of the mixture as it travels upwards. The different chemical components of the mixture travel at different rates, which results in a separation along the height of the paper. This process of allowing the solvent to travel through the paper is referred to as “
developing” the chromatogram. If the chemical substances happen to be colored species, then they can be easily seen as different colored spots or streaks along the paper.

The physical property which results in the separation during any chromatography experiment is the preference for a substance within the mixture to stay in one
phase more than in the other phase. In the specific case of paper chromatography, the two phases are the paper phase (i.e., when the substance is adsorbed onto the paper) and the solvent phase (i.e., when the substance is dissolved in the solvent). The reason why a substance would prefer one phase or the other lies in its chemical structure and corresponding physical properties. For example, chemicals which are
hydrophilic (polar substances which are attracted to water or “water-loving”) would behave differently from chemicals which are
hydrophobic (nonpolar substances which avoid water or “water-fearing”). The hydrophilic chemicals would be expected to move up the paper much more rapidly than hydrophobic chemicals if water was used as the developing solvent.

Who Kidnapped Fluffy?!!

Scenario: You are a Chemical Analyst assigned the task of identifying ink from a ransom note left at the scene of the kidnapping. Fluffy, a valuable pet Persian kitty has been swiped, and the thieves left a hand-written note using some sort of black ink pen. In the room where the note was left, several different black ink pens were also found. The detectives have obtained unique fingerprints from each of these pens. They are hoping that, if the correct pen can be identified, then the corresponding fingerprints can be used to identify the thief. Your mission is to determine which ink pen was used!

The Procedure used to develop the chromatograms was:

1. 4 strips of chromatography paper were dotted with ink in the bottom center of each paper. The central dot corresponds to the ink used in the ransom note.

2. A ruler and a
pencil were used to
draw a line through the unknown ink dot on 4 strips of chromatography paper. Near the very top of the opposite end of the paper, “Acetone” was written on two of the strips and “Alcohol” was written on the other two strips. The strips were also dotted using the pens found at the abduction site. Those pens were labeled “1,” “2,” “3,” and “4.” The numbers corresponding to the pens found at the crime scene were marked on the chromatography paper using a
pencil.

3. Two different solvent systems (acetone/water and isopropyl alcohol/water) were prepared.

4. Two chromatography papers, labeled “Acetone,” were placed in the beakers containing the acetone/water solvent mixture
with the ink dot edge at the bottom. The other two chromatography papers, labeled “Alcohol,” were placed in the beakers containing the isopropyl alcohol/water solvent mixture
with the ink dot edge at the bottom.

5. The chromatograms were developed. Watch the video linked in Falcon Online Content to see the procedure AND the data!

image3

image4

image1

image2